Author: profacgen

Protein engineering has revolutionized the way we understand and interact with the building blocks of life. One of the most exciting advances in this field is in vitro glyco engineering, a technique that allows for the precise modification of proteins’ sugar molecules, or glycans. This article will delve into the world of in vitro glyco engineering (IVGE), exploring its principles, applications, and the incredible potential it holds for protein design. What is In Vitro Glycoengineering? Before we dive into the details, it’s essential to understand the basics. Glyco engineering is the process of modifying a protein’s glycans, which are complex carbohydrate molecules…

Cellular signal transduction pathways are complex networks that allow cells to respond to their environment, with phosphorylation playing a central role in these processes. The ability to detect phosphorylation in a genetic level is crucial for understanding how these pathways function in both healthy and diseased states. Chromatin immunoprecipitation (ChIP) has emerged as a powerful technique for the analysis of protein-DNA interactions, including the study of phosphorylation events within signal transduction pathways. Principles of ChIP-Based Phosphorylation Detection ChIP is based on the principle of immunoprecipitating chromatin fragments using antibodies specific to proteins of interest. These proteins may be transcription factors,…